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Coxsackievirus A9 has been crystallized as small rhombic dodecahedra of maximum dimension 0.3 mm. These crystals have been shown, using synchrotron radiation, to diffract X-rays to beyond 3 A, and to have a stability in the beam comparable to that of other related virus crystals. The unit cell is tetragonal with dimensions a = b = 495 A, c = 695 A and alpha = beta = gamma = 90 degrees, with a space group of P4n22. A substantial body of diffraction data has been collected and this crystal form appears to be suitable for structure determination. Phasing of these data will be attempted using molecular replacement.

Original publication

DOI

10.1006/jmbi.1993.1179

Type

Journal article

Journal

J Mol Biol

Publication Date

20/03/1993

Volume

230

Pages

667 - 669

Keywords

Animals, Cell Line, Crystallization, Enterovirus, X-Ray Diffraction