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Six structures of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) containing combinations of resistance mutations for zidovudine (AZT) (M41L and T215Y) or lamivudine (M184V) have been determined as inhibitor complexes. Minimal conformational changes in the polymerase or nonnucleoside RT inhibitor sites compared to the mutant RTMC (D67N, K70R, T215F, and K219N) are observed, indicating that such changes may occur only with certain combinations of mutations. Model building M41L and T215Y into HIV-1 RT-DNA and docking in ATP that is utilized in the pyrophosphorolysis reaction for AZT resistance indicates that some conformational rearrangement appears necessary in RT for ATP to interact simultaneously with the M41L and T215Y mutations.

Original publication

DOI

10.1128/jvi.76.19.10015-10019.2002

Type

Journal article

Journal

J Virol

Publication Date

10/2002

Volume

76

Pages

10015 - 10019

Keywords

Adenosine Triphosphate, Anti-HIV Agents, Codon, Crystallization, Drug Resistance, Viral, HIV Reverse Transcriptase, Lamivudine, Mutation, Protein Conformation, Zidovudine