With extensive technological and computational leaps over the past couple of decades, cryo-electron microscopy (cryo-EM) has rapidly come to the fore as a workhorse of structural biology, enabling the imaging of particles, including protein complexes, viruses and cells, in their native state at resolutions that supersedes those of other imaging techniques.
OPIC offers a range of plunging devices to cater for a range of experimental constraints, be it the need for single-sided blotting, or plunge freezing of high containment level organisms.
Within our 'yellow suite', operating at category 2 level (with category 3 capability), we have three plunging devices:
-CryoPlunge-3 (CP3, Gatan).
In addition, we have two Vitrobots.
OPIC houses two state-of-the-art cryo-electron microscopes to both internal and external users:
Titan Krios G3i (Thermo Fisher Scientific) 300-kV transmission electron microscope equipped with Falcon III and K2 Summit/GIF (Gatan) direction electron detectors.
Glacios (Thermo Fisher Scientific) 200-kV, equipped with a Falcon III.
Cell culture for virus production
OPIC houses two SAPO category 3 level laboratory facilities for mammalian cell culture for virus infection, production and purification.